| IF: 27.7 Article: Gut-microbiome-expressed 3β-hydroxysteroid dehydrogenase degrades estradiol and is linked to depression in premenopausal females. Journal: Cell Metabolism Author: Di Li, Ting Sun, Yongqing Tong, Juan Le, Qian Yao, Jun Tao, Hang Liu, Woer Jiao, Yufeng Mei, Jingru Chen, Zhongchun Liu, Gaohua Wang, Yan Li Insititution/University: Wuhan University Renmin Hospital DOI: 10.1016/j.cmet.2023.02.017 Abstract: Estradiol decline can result in depressive disorders in females; nevertheless, the causes of this decline are unclear. In this study, we isolated estradiol-degrading Klebsiella aerogenes from the feces of premenopausal females with depression. In mice, gavaging with this strain led to estradiol decline and depression-like behaviors. The gene encoding the estradiol-degrading enzyme in K. aerogenes was identified as 3β-hydroxysteroid dehydrogenase (3β-HSD). Heterologously expressing 3β-HSD resulted in Escherichia coli obtaining the ability to degrade estradiol. Gavaging mice with 3β-HSD-expressing E. coli decreased their serum estradiol levels, causing depression-like behaviors. The prevalence of K. aerogene and 3β-HSD was higher in premenopausal women with depression than in those without depression. These results suggest that the estradiol-degrading bacteria and 3β-HSD enzymes are potential intervention targets for depression treatment in premenopausal women. Keywords: 3β-hydroxysteroid dehydrogenase; depression; estradiol; gut; microbes. |
 |
| Catalog No. | Product Name |
| JL12087 | Mouse 5-Hydroxytryptamine (5-HT) ELISA Kit |
| JL50852 | Mouse Estradiol (E2) ELISA Kit |